These organisms use photosynthesis to generate biomass from light and carbon dioxide and include plants, mosses, macroalgae, microalgae, cyanobacteria and purple bacteria. In tissue engineering bioreactors can be used to aid in the in vitro. Environmental conditions in the reactor vessel play a major role in the reproduction and growth of microbial cells7. Modelling and optimization of combined wastewater treatment. Peng yu, thong see lee, yan zeng and hong tong low, fluid dynamics and oxygen transport in a micro bioreactor with a tissue engineering scaffold, international journal of heat and mass transfer, 10. Regulation of cell function in tissue engineering currently, the regulation of cell function and the growth of threedimensional tissues are major areas of focus in developing new tissue engineering techniques. The engineering of 3d tissue constructs include cell maintenance, proliferation and tissue formation. Faculty of science and engineering department of chemical engineering modelling and optimization of combined wastewater treatment and co 2 biofixation in a batch algal photobioreactor ahmed majeed daife al ketife this thesis is presented for the degree of doctor of philosophy of curtin university may 2017. The function of the bioreactor is to provide a suitable environment in. State of the art and integrated process design for. Bioreactor advances functional tissue engineering mddi.
Bergstrom, investigation of the in vitro culture process for skeletaltissueengineered constructs using computational fluid dynamics and experimental methods, journal of biomechanical engineering, 10. A photobioreactor is a bioreactor that utilizes a light source to cultivate phototrophic microorganisms. An overview of culture systems and bioreactors used for these purposes is shown in fig. Request pdf principles of bioreactor design for tissue engineering tissue engineering bioreactors apply engineering concepts to mimic native tissue environment and function, and study cellular. Bioreactors and products of bioreaction engineering essay. Besides its specific aeration systems vogelbusch has the expertise in selecting the appropriate components and proven knowhow, to run fermentations stable.
Photobioreactor design for improved energy efficiency of microalgae production a thesis presented to the faculty of california polytechnic state university, san luis obispo in partial fulfillment of the requirements for the degree master of science in biochemical engineering by alexander burns december 2014. Getting nerve cells to grow in the lab can be a challenge. Ethanol and butanol fermentation research focuses on insitu product removal and process compression, as well as on reactor design and mathematical modeling. Advances in the design of bioreactor systems bioreactors are closed systems in which a biological process can be carried out under controlled, environmental conditions. Considerations for photobioreactor design and operation. Tissueengineering bioreactors apply engineering concepts to mimic native tissue environment and function, and study cellular responses under biologically sound conditions. Photobioreactor allows much higher growth rates and purity levels than anywhere in natural or habitats similar to nature. A bioreactor can be defined as a device that uses mechanical means to influence biological processes.
My research group is loosely structured in 5 research clusters. The gap in time between the plants reveals that some of the design features have undergone changes, while others are unchanged. Biotechnology academy practical bioreactor training. Design principles of photobioreactors for cultivation of. Designing a bioreactor is complex engineering challenge. Within weeks the bone tissue engineered scaffold is ready to be implanted into the patients body. The 2 mm opening at the base of the c ring established a differential.
Scale related work investigate key process parameters from microscale, via benchscale to pilotscale. Shear stress a bone tissue engineering strategy involving the in vitro expansion of cells on a scaffold before implantation into the body represents a promising alternative to current clinical treatments. The decisions made in the design of the bioreactor might have a significant impact on overall process performance. Bioreactors are essential in tissue engineering, not only because they provide an in vitro environment mimicking in vivo conditions for the growth of tissue substitutes, but also because they enable systematic studies of the responses of living tissues to various mechanical and biochemical cues. Microalgae have great biotechnological potential for production of substances through photosynthesis. Msc in biochemical engineering followed by 23 years fermentation experience,14 years in process development and protein supply, 2 years in fermenter sales and technical support and 7 years as owner of biomax scientific consultancy.
Developments in tissue engineering for human medicine are increasing rapidly. The role of perfusion bioreactors in bone tissue engineering. A mathematical model was developed for predicting the rate of algal biomass accumulation, nutrient re and light intensity profile i av against a range of nutrient. The research focuses on investigation and optimization using box behnken design of the key parameters of co2 gas concentration, light intensity, temperature, feedwater nutrient concentration, and wastewater origin municipal primary and secondary, and petroleum industry on photobioreactor algal growth parameters. The first aim of this study is to designing a bioreactor for bone tissue engineering. Ijedr1401215 international journal of engineering development and research. Design principles of photobioreactors for cultivation of microalgae the present hype in microalgae biotechnology has shown that the topic of photobioreactors has to be revisited with respect to availability in really large scale measured in hectars footprint area, minimization of cost, auxiliary energy demand as well as maintenance and life span. This chapter has not detailed all of the issues related to tissue engineering bioreactors.
Photobioreactor or pbr, is the controlled supply of specific environmental conditions for respective species. The bioreactor is designed for studying the tissue engineering process in 1 cm3 cubic scaffolds and allows for realtime monitoring the process. To design a bioreactor, some objectives have to be defined. Author links open overlay panel ralf portner a stephanie nagelheyer a christiane goepfert a peter adamietz b norbert m. With respect to tissue engineering, bioreactors are used for different purposes such as i cell proliferation on a small scale e. Nanoliter scale microbioreactor array for quantitative. Advances in stem cell biology, biomaterials science and scaffold design underpin this emerging science. Martin tampier, envint consulting eric bibeau, university of manitoba january 14, 2009. Effects of potential fermentation inhibitors are quantified in miniaturized highthroughput experiments. To improve in vitro culture, bioreactor systems have been widely researched for bone tissue engineering purposes. Box behnken design bbd was used to identify the optimum cultivation condition, based on nutrient re from wastewater with r c in a single practical experimental study. Photobioreactor design for improved energy efficiency of. A tissue engineering approach involves the implantation in the site of injury of a 3d porous biodegradable structure seeded with osteoblastic or mesenchymal stem cells, which are one valuable cell source for orthopedic applications and its growth rate and osteogenic differentiation have been studied for years. Bioreactor system design crc press book describes the stateoftheart techniques and methods involved in the design, operation, preparation and containment of bioreactor systems, taking into account the interrelated effects of variables associated with both upstream and downstream stages of the design process.
Adherentcell products proteins, cell therapies process focus. Design of bioreactor systems thanks to its extensive experience with microbial production techniques, vogelbusch is well acquainted with the differing requirements of designing bioreactors. In this article we describe the design, construction, and implementation of a novel photobio reactor system for the cultivation of photoautotrophic cells. What you see growing in the petri dish shown above are nerve cells from an embryonic rat. An equally important facet of this field is the rational design and operation of bioreactors to control the nascent tissue growth. Korossis and others published bioreactors in tissue engineering find, read and cite all the research you need on researchgate. Biomass pretreatment thermal, chemical ionic liquids, mechanical and biological pretreatment methods for lignocellulosic biomass are investigated. Design of a stackedlayer tubular photobioreactor for microalgae cultivation varit kunopagarnwong, thongchai rohitatisha srinophakun department of chemical engineering, faculty of engineering, kasetsart university, bangkok, thailand theerawat sritaweewat. Biotechnology academy practical bioreactor training courses. Lowpressure polymeric membrane mixed liquor permeate mlss 5,000 16,000 mgl flow bod for bioreactor design. The surface should be well suited for cell attachment.
Choice of favourable materials for pbr design as illustrated in the previous section, a range of materials is available for photobioreactor construction which not only possesses significant benefits but also show a few disadvantages. Enduratec minneapolis has developed what it believes is the first commercially available intelligent bioreactor for functional tissue engineering. Oct 12, 2003 tissue engineered products are expected to play a leading role in cardiovascular and orthopedic tissue repair and replacement. Cell maintenance and proliferation is usually performed in culture systems developed for the monolayer culture of adherent cells tflasks, petri dishes, multi well plates. In the interest of pursuing further research into vocal fold cells, a bioreactor was constructed to effectively simulate the environment of the vocal fold tissue within the human body. Development of a bioreactor for bone tissue engineering. The importance of the initial steps in the development of a bioprocess, such as strain and media.
In order to determine design criteria for tissue engineering, it is necessary to understand how. Design of a stackedlayer tubular photobioreactor for. But when it works, the result can be a thing of beauty for both science and art. Oct 01, 2012 a tissue engineering approach involves the implantation in the site of injury of a 3d porous biodegradable structure seeded with osteoblastic or mesenchymal stem cells, which are one valuable cell source for orthopedic applications and its growth rate and osteogenic differentiation have been studied for years. A new combined cell seeding and perfusion system for vascular tissue engineering ralf sodian, m. Pdf bioreactor design for oral and dental tissue engineering. A fluid dynamics approach to bioreactor design for cell and. Bioreactor design for tissue engineering sciencedirect. A bioreactor system comprises a bioreactor, sensors and actuators, a control system and software to monitor and control the conditions inside the bioreactor. For the first time in a single volume, the design, characterisation and. Bioreactor advances functional tissue engineering mddi online. Principles of bioreactor design for tissue engineering. Describes the stateoftheart techniques and methods involved in the design, operation, preparation and containment of bioreactor systems, taking into account the interrelated effects of variables associated with both upstream and downstream stages of the design process. Jul 29, 2006 bioreactors are essential in tissue engineering, not only because they provide an in vitro environment mimicking in vivo conditions for the growth of tissue substitutes, but also because they enable systematic studies of the responses of living tissues to various mechanical and biochemical cues.
Among the types of glasses, borosilicate pyrex glass is commonly used in the solar hot water collector of pbr. Light capture process and electron transportation imply energy losses due to reflection, fluorescence emission, and energy dissipation as heat, giving a maximum theoretical value of 8. Knowledge of reaction kinetics is essential for understanding how a biological reactor works. Bioreactor design for tissue engineering request pdf. The open raceway pond is the primary type of reactor considered. It is thus critical to select the best material that satisfies all the criteria of an efficient pbr design. Postdoctoral research in tissue engineering of human skin. Disclaimer and safety precautions provides the science fair project ideas for informational purposes only. Within the artificial environment of a photobioreactor, specific conditions are carefully. These reactors are used to carry out chemical process of organisms, often these reactors are made of stainless steel. In particular, tissue engineering has adopted bioreactors for the scalable reproducible production of biological constructs. A fluid dynamics approach to bioreactor design for cell. Tissue engineering bioreactors apply engineering concepts to mimic native tissue environment and function, and study cellular responses under biologically sound conditions. Tissueengineered products are expected to play a leading role in cardiovascular and orthopedic tissue repair and replacement.
498 96 919 1443 113 247 1120 1333 1358 593 303 377 46 1282 951 1045 1382 339 760 738 667 151 1416 1162 3 313 890 304 856 1449 992 1350 669 1058 512 1309 1323 1122 989 963 668 1445